Plasmids, which possess genes that are resistant to antibiotics, are used by microbes as a defense mechanism. In nature, plasmids can be transferred to different types of host cells, spreading antibiotic resistance there. The resistance to antibiotics by a number of microorganisms has become a public health concern recently. In the lab, plasmids are used in genetic engineering of microorganisms. With this lab activity that can be completed in two 60 minutes sessions, students electrophorese plasmid pBR322, natural and restricted, to determine fragment length in nucleotide base pairs, then determine if the plasmids’ structure is linear, supercoiled, or open-circular based on their electrophoretic mobility. The kit includes enough materials to run eight gels, a teacher’s guide, and student copymaster. An electrophoresis chamber, power supply, and 10 μL micropipets, required, are available separately. Note: Coupon included for perishable materials. Redeem by mail, fax, phone, or e-mail.
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