Paternity was once determined by blood type, based on the concept that a child’s blood type depends on the blood type of the parents. Yet blood type can only exclude possible biological parents, not definitively determine the identity of the real parents. With DNA fingerprinting, however, it is possible to establish familial relationships with a high degree of certainty. In this lab activity, students work with a real-life situation, electrophoresing nonhuman DNA samples, simulating those of a child, mother, and two possible fathers; compare the banding patterns; and determine which of the two men is the most probable match to be the child’s biological father. The activity can be completed in two 60 minutes sessions and comes with enough materials to run eight gels, a teacher’s guide, and student copymaster. An electrophoresis chamber, power supply, 10 μL micropipets, and UV transilluminator (for ethidium bromide kit only) required, are available separately. Note: Coupon included for perishable materials. Redeem by mail, fax, phone, or e-mail. Ethidium bromide version sold only to colleges, universities, and accredited laboratories. Check your local regulations for disposal of ethidium bromide.
Materials Included
•200 mL Agarose, 0.8%, 500 mL 5X TBE running buffer
•1 Bottle, of WARD’S QUIKView DNA stain concentrate, 60 mL (36 W 8551)
•1 Tube of Ethidium Bromide DNA stain, 50 ?L (36 W 9951)
•8 Gel staining trays
•1 Biohazard bag (36 W 9951)
•1 Redemption coupon for the following DNA samples: Mother, 80 ?L, Child, 80 ?L, Alleged father #1, 80 ?L, Alleged father #2, 80 ?L
Materials Needed But Not Provided
•Agarose electrophoresis chambers
•Power supplies
•Waterbath or microwave
•Micropipets with tips
•Gloves
•Apron
•Goggles
•Lab markers
•UV transilluminator (36 W 9951)
•UV goggles (36 W9951)
Time Requirement
•Casting gels and diluting buffer: 30 minutes
•Loading and running gel: 60 minutes
•Staining and analyzing gel: 60 minutes
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