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WARD’S Human Fingerprinting by PCR Lab Activity |
| Investigate the Various Uses of DNA Polymorphism Identification in Individuals |
Certain DNA basepair sequences vary from person to person; for example, in one person a particular basepair sequence may be repeated ten times, while in another person the same sequence may be repeated 25 times. Known as polymorphisms, the repetitions are an identifying factor in a person’s DNA, and thereby can be used for DNA fingerprinting in forensics, paternity testing, and disease diagnosis. The basepair sequences can be separated using electrophoresis and the resulting banding pattern used to distinguish one person from the next, which students perform with the lab activity. Using DNA isolated from their own cheek cells, students amplify a variable DNA region known as a D1S80 locus, electrophorese the DNA, and identify whether the student is homozygous or heterozygous for the D1S80 locus. The kit includes enough materials to run 24 PCR reactions, a teacher’s guide, and student copymaster. Note: Coupon included for perishable materials. Redeem by mail, fax, phone, or e-mail. Ethidium bromide version sold only to colleges, universities, and accredited laboratories. Check your local regulations for disposal of ethidium bromide.
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