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Products
WARD’S LC50: How Much is Too Much? Lab Activity |
A Vivid Bioassay with Results that Glow |
Students will conduct a bioassay demonstrating the toxic effects of copper sulfate (a common algaecide) on a population of Daphnia. They will feed the Daphnia a fluorescently-tagged sugar after exposing them to various concentrations of copper sulfate. Only the healthy Daphnia will glow under long-wave UV light. Students will then pool their data to predict the lethal concentration necessary to cause 50% mortality and arrive at an overall LC50 value for the entire class. In addition, a demonstration of serial dilutions using colored water will mimic the toxic pollutants found in the same low concentrations (ppm) used for the copper sulfate. The kit contains a teacher’s guide, student copymaster, and enough materials for 15 setups.
Note: Coupon included for perishable materials. Redeem by mail, fax, phone, or e-mail. This product is protected by U.S. Patent # 5,094,944. Foreign patents are pending.
Materials Included
•3 Daphnia magna cultures
•500 mL CuSO4 solution (0.5 ppm)
•1 Tagged sugar vial
•130 Medicine cups
•75 Disposable pipets
•1 Vial, food coloring
•15 Plastic vials and caps
•13 Plastic cups
Materials Needed But Not Provided
•UV Light or “black light”
•UV Protective goggles
•Beaker or small container
•Distilled water
•Scissors
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