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WARD’S Introduction to Biotechnology: DNA Extraction Lab Activity |
| Examine the Physical Properties of Onion DNA |
A specially prepared onion lysate solution allows students to easily extract and view DNA. A macromolecular solution, which has similar characteristics to DNA, is also included for comparison of long, linear-chain molecules. The class-size activity comes with everything you need to perform the lab but an onion. The demonstration size comes with an onion lysate already prepared. The lab activity may be completed in 15 minutes or less. The demonstration size comes with enough materials to perform five extractions, while the class size comes with enough materials for six setups, a teacher’s guide, and student copymaster.
Materials Included •1 Jar of macromolecular solution •4 Bottles of ethanol, 30 mL •15 Test tubes •1 Bottle of detergent/salt powder •15 DNA spoolers •15 Plastic pipets •1 Package of cheesecloth
Materials Needed But Not Provided •Ice water bath •Hot water bath •Beaker, 500 ml •Large onion
Time Requirement •Activity 1: 30 minutes •Activity 2: 45 minutes
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