Splicing pieces of DNA together to create a recombinant DNA molecule is used in numerous real-life applications in the fields of food production, agriculture, pharmaceutical production, and treatment of genetic diseases, such as producing nutrient supplements and insulin. In this lab activity, students reconstruct a DNA strand by joining individual fragments that have been restricted with an endonuclease, then test their results by running a gel. The entire activity, including staining, can be completed in one 90-minute lab session, or it can be divided into two 45-minute lab sessions. It comes with enough materials for eight ligation procedures, a teacher’s guide, and student copymaster. An electrophoresis chamber, power supply, and 1–5 μL micropipets, required, are available separately. Note: Coupon included for perishable materials. Redeem by mail, fax, phone, or e-mail.
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